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This study was carried out to obtain the basic data for artificial culture of Grifola umbellata. The optimal condition for the mycelial growth was obtained at 20℃ and pH 4, respectively. G. umbelleta showed the most favorable growth on the Hoppkins media. Carbon sources such as glucose, fluctose and manitol were favorable for stimulating a mycelial growth of G. umbellata. Valine, one of nitrogen sources also appeared to be favorable to a mycelial growth. 1fie optimum C/N ratio was about 30:1 in case that 1 % glucose as carbon source was mixed with the basal media. Lactic acid as organic acid was most favorable to the mycelial growth. Also, thiamine-Hcl as vitamin source was favorable. The mineral nutrient of FeSO₄ or MgSO₄ was most favorable to G. umbellata, and its optimal concentration was about 0.01% in FeSO₄ and 0.1% in MgSO₄, respectively. Among 4 different cereal extract media, polished rice extract medium which was mixed with silkworm pupae was most suitable for a favorable growth of G. umbedlata.
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